trap solution (Nikon)
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Trap Solution, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trap+solution/ECLIPSE+Ts2R/pmc12655559-63-45-59
Average 99 stars, based on 1626 article reviews
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Cell Culture:Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 104 cells/well in 96-well plates, and cells were cultured at 37 ◦C in 5% CO2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 10 4 cells/well in 96-well plates, and cells were cultured at 37 °C in 5% CO 2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a Staining:Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 104 cells/well in 96-well plates, and cells were cultured at 37 ◦C in 5% CO2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 10 4 cells/well in 96-well plates, and cells were cultured at 37 °C in 5% CO 2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a Article Title: Cell cytoskeleton and proliferation study for the RANKL‐induced RAW264.7 differentiation Article Snippet: .. Subsequently, both cell lineages were washed by PBS and then stained by Microscopy:Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 104 cells/well in 96-well plates, and cells were cultured at 37 ◦C in 5% CO2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 10 4 cells/well in 96-well plates, and cells were cultured at 37 °C in 5% CO 2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a Software:Article Title: Cell cytoskeleton and proliferation study for the RANKL‐induced RAW264.7 differentiation Article Snippet: .. Subsequently, both cell lineages were washed by PBS and then stained by |
