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trap solution  (Nikon)


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    Structured Review

    Nikon trap solution
    Trap Solution, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trap+solution/ECLIPSE+Ts2R/pmc12655559-63-45-59
    Average 99 stars, based on 1626 article reviews
    trap solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 104 cells/well in 96-well plates, and cells were cultured at 37 ◦C in 5% CO2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 10 4 cells/well in 96-well plates, and cells were cultured at 37 °C in 5% CO 2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).

    Staining:

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 104 cells/well in 96-well plates, and cells were cultured at 37 ◦C in 5% CO2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 10 4 cells/well in 96-well plates, and cells were cultured at 37 °C in 5% CO 2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).

    Article Title: Cell cytoskeleton and proliferation study for the RANKL‐induced RAW264.7 differentiation
    Article Snippet: .. Subsequently, both cell lineages were washed by PBS and then stained by TRAP solution at 37°C in 1 hour, the staining results were evaluated by Nikon (Nikon) and NIS‐Element analysis software (Nikon) for image analysis. ..

    Microscopy:

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 104 cells/well in 96-well plates, and cells were cultured at 37 ◦C in 5% CO2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).

    Article Title: Generation and Purification of RANKL-Derived Small-Fragment Variants for Osteoclast Inhibition
    Article Snippet: For the tartrate-resistant acid phosphatase (TRAP) assay, bone marrow macrophages (BMMs) were flushed from the femurs of 6-week-old female Balb/c mice and cultured in alpha-MEM containing 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) and 30 ng/mL macrophage colony-stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) after treatment with ACK lysis buffer (Gibco, Gaithersburg, MD, USA). .. The BMMs were plated at a density of 1.4 × 10 4 cells/well in 96-well plates, and cells were cultured at 37 °C in 5% CO 2 for 4 d. Subsequently, the cells were fixed with 10% formalin for 20 min and stained with a TRAP solution for 30 min. Multinucleated osteoclasts were observed using an ECLIPSE Ts2R microscope (Nikon, Tokyo, Japan). .. The ratio of the resorbed to total area was calculated using ImageJ analytical software (Version 1.54k http://rsbweb.nih.gov/ij/ (accessed on 17 February 2025); National Institutes of Health, Bethesda, MD, USA).

    Software:

    Article Title: Cell cytoskeleton and proliferation study for the RANKL‐induced RAW264.7 differentiation
    Article Snippet: .. Subsequently, both cell lineages were washed by PBS and then stained by TRAP solution at 37°C in 1 hour, the staining results were evaluated by Nikon (Nikon) and NIS‐Element analysis software (Nikon) for image analysis. ..



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    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative <t>immunohistochemical</t> <t>staining</t> images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative <t>TRAP</t> staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.
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    Image Search Results


    Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Journal: Bioactive Materials

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1

    doi: 10.1016/j.bioactmat.2025.10.040

    Figure Lengend Snippet: Effect of NGF-G/P@Yoda1 on promoting bone regeneration and bone remodeling in femur def ect model. (A–D) Representative immunohistochemical staining images at week 8 for RUNX2 and OPN and semi-quantitative analysis of positive areas to evaluate the osteogenic capacity of composite hydrogels. (E, F) Representative immunofluorescence (IF) staining images at week 8 of OCN and semi-quantitative analysis. (G–J) Representative IF staining images at week 8 of YAP1and β-catenin and semi-quantitative analyses. (K, L) Representative TRAP staining images at week 8 and semi-quantitative analysis of positive areas to assess bone resorption and remodeling. The quantitative data (n ≥ 3) in the figures represent as the averages ± SD, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

    Article Snippet: Following dewaxing and rehydration of femur sections, they were treated with TRAP staining solution (G105, Servicebio, Wuhan, China) at 37 °C for 30 min in the absence of light.

    Techniques: Immunohistochemical staining, Staining, Immunofluorescence